Proteomic and Bioinformatic Investigations of Heat-Treated Anisakis simplex Third-Stage Larvae

Third-stage larvae of Anisakis simplex (Rudolphi, 1809) in the Great Cormorant [Phalacrocorax carbo sinensins (Blumenbach, 1798)] from the Vistula Lagoon, Poland

Five out of 28 Great Cormorants collected in spring 2005 from the Vistula Lagoon were found to contain a total of 19 (16 in the stomach and 3 in the intestine) stage III larvae of Anisakis simplex. Spring is the season when nematode-infected herring from the western Baltic, Danish straits, and the North Sea arrive in the Vistula Lagoon and transmit the infection to cormorants feeding on them. T...

Anisakis simplex: current knowledge.

Anisakiasis, firstly described in 1960s in the Netherlands, is a fish-borne parasitic disease caused by the consumption of raw or undercooked fish or cephalopods contaminated by third stage (13) larvae of the Anisakidae family, in particular Anisakis simplex (As), A. pegreffii and Pseudoterranova decipiens. Every year, approximately 20,000 cases of anisakiasis were reported worldwide, over 90% ...

Anisakis simplex in Norway

Anisakis simplex: CO(2)-fixing enzymes and development throughout the in vitro cultivation from third larval stage to adult.

We studied the effect of CO(2) on the in vitro cultivation of Anisakis simplex, an aquatic parasitic nematode of cetaceans (final hosts) and fish, squid, crustaceans and other invertebrates (intermediate/paratenic hosts), and, occasionally, of man (accidental host). The results showed that a high pCO(2), at a suitable temperature, is vital for the optimum development of these nematodes, at leas...

Expression and Characterization of α-Methylacyl CoA Racemase from Anisakis simplex Larvae

Larval excretory-secretory products of Anisakis simplex are known to cause allergic reactions in humans. A cDNA library of A. simplex 3rd-stage larvae (L3) was immunoscreened with polyclonal rabbit serum raised against A. simplex L3 excretory-secretory products to identify an antigen that elicits the immune response. One cDNA clone, designated as α-methylacyl CoA racemase (Amacr) contained a 1,...