Purification and Characterization of Glyceraldehyde-3-phosphate Dehydrogenase from European Pilchard Sardina pilchardus
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چکیده
منابع مشابه
Purification and characterization of glyceraldehyde-3-phosphate dehydrogenase from European pilchard Sardina pilchardus.
The NAD+-dependent cytosolic glyceralehyde-3-phosphate dehydrogenase (GAPDH; EC 1.2.1.12) was purified from the skeletal muscle of European pilchard Sardina pilchardus and its physicochemical and kinetic properties were investigated. The purification method consisted of two steps, ammonium sulfate fractionation followed by Blue Sepharose CL-6B chromatography, resulting in an approximately 78-fo...
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The NAD(+)-dependent cytosolic glyceraldehyde-3-phosphate dehydrogenase (GAPDH, EC 1.2.1.12), which is recognized as a key to central carbon metabolism in glycolysis and gluconeogenesis and as an important allozymic polymorphic biomarker, was purified from muscles of two marine species: the skeletal muscle of Sardina pilchardus Walbaum (Teleost, Clupeida) and the incompressible arm muscle of Oc...
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ژورنال
عنوان ژورنال: Acta Biochimica et Biophysica Sinica
سال: 2007
ISSN: 1672-9145,1745-7270
DOI: 10.1111/j.1745-7270.2007.00357.x