Purification and cloning of a proline 3-hydroxylase, a novel enzyme which hydroxylates free L-proline to cis-3-hydroxy-L-proline
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منابع مشابه
Purification and cloning of a proline 3-hydroxylase, a novel enzyme which hydroxylates free L-proline to cis-3-hydroxy-L-proline.
Proline 3-hydroxylase was purified from Streptomyces sp. strain TH1, and its structural gene was cloned. The purified enzyme hydroxylated free L-proline to cis-3-hydroxy-L-proline and showed properties of a 2-oxoglutarate-dependent dioxygenase (H. Mori, T. Shibasaki, Y. Uosaki, K. Ochiai, and A. Ozaki, Appl. Environ. Microbiol, 62:1903-1907, 1996). The molecular mass of the purified enzyme was ...
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26 Gerhart, J. C., and A. B. Pardee, in Synthesis and Structure of Macromolecules, Cold Spring Harbor Symposia on Quantitative Biology, vol. 28 (1963), p. 491. 27 Changeux, J. P., in Subunit Structure of Proteins, Brookhaven Symposia in Biology, no. 17 (1964), p. 232. 28 Antonini, E., J. Wyman, M. Brunori, J. F. Taylor, A. Rossi-Fanelli, and A. Caputo, J. Biol. Chem., 239, 907 (1964). 29 Gibson...
متن کاملFunctional characterization of aconitase X as a cis-3-hydroxy-L-proline dehydratase
In the aconitase superfamily, which includes the archetypical aconitase, homoaconitase, and isopropylmalate isomerase, only aconitase X is not functionally annotated. The corresponding gene (LhpI) was often located within the bacterial gene cluster involved in L-hydroxyproline metabolism. Screening of a library of (hydroxy)proline analogues revealed that this protein catalyzes the dehydration o...
متن کاملA new method for isolation of hydroxy-L-proline and L-proline from gelatin.
Several investigators have reported that commercial L-proline is contaminated with small amounts of hydroxy-n-proline (l-3). During the course of investigations in this laboratory all of five commercial samples proved to contain up to 3.5 per cent hydroxyproline when tested by the method of Neuman and Logan (1). The preparation of n-proline from gelatin by the formation of complex salts is tedi...
متن کاملEpimerization of trans-4-hydroxy-L-proline to cis-4-hydroxy-D-proline during acid hydrolysis of collagen.
1. trans-4-Hydroxy-r,-proline is epimerized to &+hydroxy-n-proline under the conditions generally used for the hydrolysis of proteins for analyses of amino acids. 2. The cis epimer elutes from a column of UR-30 resin on a Beckman amino acid analyzer at the same time as threonine and hence would be undetected. 3. About 8% of the trans-4-hydroxy-L-proline in collagen can be epimerized to c&4-hydr...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1997
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.179.18.5677-5683.1997