Purification of an Exopolygalacturonase fromPenicillium viridicatum RFC3Produced in Submerged Fermentation
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چکیده
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Purification of an Exopolygalacturonase from Penicillium viridicatum RFC3 Produced in Submerged Fermentation
An exo-PG obtained from Penicillium viridicatum in submerged fermentation was purified to homogeneity. The apparent molecular weight of the enzyme was 92 kDa, optimum pH and temperature for activity were pH 5 and 50-55 degrees C. The exo-PG showed a profile of an exo-polygalacturonase, releasing galacturonic acid by hydrolysis of pectin with a high degree of esterification (D.E.). Ions Ca(2+) e...
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Pectate lyase (PL) was produced by the filamentous fungus Penicillium viridicatum RFC3 in solid-state cultures of a mixture of orange bagasse and wheat bran (1 : 1 w/w), or orange bagasse, wheat bran and sugarcane bagasse (1 : 1 : 0.5 w/w), and in a submerged liquid culture with orange bagasse and wheat bran (3%) as the carbon source. PL production was highest (1,500 U mL(-1) or 300 Ug(-1) of s...
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For the successful operation of aerobic fermentations it is essential to supply the microorganiisms with sufficient oxygen to meet their requirements at any stage in the process. Failure to supply enough oxygen may lead to undesirable changes in enzymatic makeup (Rolinson, 1952) or death of the organisms (Hromatka et al., 1951), with a subsequent lower yield of the desired product. In general, ...
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An investigationi was undertaken to determine the oxygen absorption rates of a submerged culture of Bacillus anthracis at all stages of its growth cycle. The determination of oxygen absorption rates of a pathogenic fermentation such as this by analysis of the exhaust gases seemed to present fewer technical difficulties than did those methods that depended upon polarographic determination of dis...
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ژورنال
عنوان ژورنال: International Journal of Microbiology
سال: 2009
ISSN: 1687-918X,1687-9198
DOI: 10.1155/2009/631942