Purification of Euglena gracilis chloroplast elongation factor Ts.
نویسندگان
چکیده
منابع مشابه
Complete sequence of Euglena gracilis chloroplast DNA.
We report the complete DNA sequence of the Euglena gracilis, Pringsheim strain Z chloroplast genome. This circular DNA is 143,170 bp, counting only one copy of a 54 bp tandem repeat sequence that is present in variable copy number within a single culture. The overall organization of the genome involves a tandem array of three complete and one partial ribosomal RNA operons, and a large single co...
متن کاملCircular chloroplast DNA from Euglena gracilis.
Chloroplast DNA of the protozoan flagellate, Euglena gracilis, exists as circular molecules, 40 mum in contour length, as shown by electron microscopy and buoyant density analyses.
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The role of light in nalidixic acid bleaching of Euglena gracilis var. bacillaris was investigated. The kinetics of loss of the chloroplast-associated DNA and the sensitivity of chloroplast replication to ultraviolet light was followed during treatment with nalidixic acid. By using the mutant P(4)ZUL, and 3-(3,4-dichlorophenyl)-, 1-dimethylurea, it was demonstrated that the requirement for ligh...
متن کاملUltraviolet Inactivation of Chloroplast Formation in Synchronously Dividing Euglena Gracilis.
Ultraviolet inactivation of chloroplast formation was studied in synchronously dividing cultures of Euglena gracilis. Sensitivity to sublethal doses given at intervals throughout the cell cycle was greater just before cell division than during division. There was approximately a twofold difference in the doseresponse relationships for the periods of high and low sensitivity.
متن کاملPurification and characterization of pyruvate:NADP+ oxidoreductase in Euglena gracilis.
Pyruvate:NADP+ oxidoreductase was homogeneously purified from crude extract of Euglena gracilis. The Mr of the enzyme was estimated to be 309,000 by gel filtration. The enzyme migrated as a single protein band with Mr of 166,000 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, suggesting that the enzyme consists of two identical polypeptides. The absorption spectrum of the native e...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1981
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)43348-0