Purification of Oncornaviruses by Agglutination with Concanavalin A
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چکیده
منابع مشابه
Galactosyltransferase and concanavalin A agglutination of cells.
A correlation has been observed between concanavalin A agglutination of various cell types and the presence of surface membrane galactosyltransferase (1-O-alpha-D-Galactosyl-myo-inositol:raffinose galactosyltransferase, EC 2.4.1.67) activity. Moreover, a reduction to less than 50% of cell surface galactosyltransferase activity occurred after treatment with concanavalin A; other cell surface gly...
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Results of experiments have indicated that reduction in biological activities at high concentrations of Japanese encephalitis virus is caused by aggregates of the virus by concanavalin A. The possibility exists that the concanavalin A binding site is different from hemagglutination and antireceptor sites of Japanese encephalitis virus.
متن کاملA quantitative assay for concanavalin A-mediated cell agglutination.
A quantitative method was developed for measuring cell agglutination by the plant lectin, concanavalin A. The method, based on an assay for intercellular adhesion, determines the concanavalin A-stimulated rate of attachment of single cells to a cell layer. The rate depended on the number of single cells and the concentration of concanavalin A. Experiments on concanavalin A agglutinability of mo...
متن کاملCell agglutination mediated by concanavalin A and the dynamic state of the cell surface.
The binding of I-labelled concanavalin A (I-Con A) to the cell surface has been studied in Ehrlich ascites tumour cells (EATC) and beef erythrocytes under various conditions. The binding of concanavalin A (Con A) to the cell surface was very specific and the available binding sites were saturated within a few minutes. The amount of I-Con A bound to EATC was 4-14 x io molecules/cell at 37 °C and...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1973
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.70.5.1308