Purification of the Yeast Centromere Binding Protein CP1 and a Mutational Analysis of Its Binding Site
نویسندگان
چکیده
منابع مشابه
Genetic Analysis of the Saccharomyces Cerevisiae Centromere-Binding Protein CP1: a Thesis
CPl is a sequence-specific DNA binding protein of yeast (Saccharomyces cerevisiae) which recognizes the highly fonserved A glement I (CDEI) of yeast centromeres. We cloned and sequenced the gene encoding CPl. The gene codes for a protein of molecular weight 39,400. When expressed in E. , i coli, the CPl gene directed the synthesis of a CDEI-binding protein having the same SDS gel mobilty as pur...
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چکیده ندارد.
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The calcium binding sites of Bakers' Yeast Transketolase (TK) was elucidated by estimating the pKa values of the functional groups that bind to calcium. These pKa's were found to be 6.25 and 7.2 relating to the pKa's of the two immidazol moieties of histidine residues on the enzyme. The rate of the binding of calcium to the enzyme was obtained separately as a function of pH. Maximum values ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1989
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)81697-0