Qualitative and quantitative structural changes during pig oocyte maturation

Quantitative proteomics reveals the dynamics of protein changes during Drosophila oocyte maturation and the oocyte-to-embryo transition.

The onset of development is marked by two major, posttranscriptionally controlled, events: oocyte maturation (release of the prophase I primary arrest) and egg activation (release from the secondary meiotic arrest). Using quantitative mass spectrometry, we previously described proteome remodeling during Drosophila egg activation. Here, we describe our quantitative mass spectrometry-based analys...

Qualitative and Quantitative Changes in Proteins in Acer Platanoides L. Seeds during Maturation

Maturation of Norway maple (Acer platanoides L.) seeds produces deep physiological dormancy and resistance to desiccation. This study used two-dimensional electrophoresis to investigate the protein products of genes activated during the complex developmental process of maturation. Qualitative and quantitative changes in protein composition during maturation were tracked in this species. The mos...

Molecular mechanisms underlying pig oocyte maturation and fertilization.

Since the pig is not only an important farm animal, but also a model animal for biomedical applications, the development of reproductive technologies in this species has been very important. In vitro oocyte maturation and fertilization (IVM-IVF) are basic techniques for a number of oocyte- or embryo-related technologies. The practical aspects for pig oocyte IVM-IVF have been reviewed, while the...

Thecal vasculature and oocyte maturation during follicular atresia in the sheep and pig.

During follicular atresia in the sheep and pig there is a marked reduction in the vasculature of the theca interna underlying the membrana granulosa. In the sheep there is no reduction of thecal blood vessels adjacent to the cumulus which remains viable. By contrast, in the pig the cumulus degenerates during atresia and the underlying thecal vasculature is greatly diminished. However, despite l...

Mitochondrial Dynamics During Mouse Oocyte Maturation