Quantification of female and male Plasmodium falciparum gametocytes by reverse transcriptase quantitative PCR
نویسندگان
چکیده
منابع مشابه
Quantification of female and male Plasmodium falciparum gametocytes by reverse transcriptase quantitative PCR.
The transmission of malaria parasites depends on the presence of sexual stages (gametocytes) in the blood, making the ratio and densities of female and male gametocytes important determinants of parasite fitness. This manuscript describes the development of reverse transcriptase quantitative PCR (RT-qPCR) assays to separately quantify mature female and male gametocytes of the human malaria para...
متن کاملMale and female P. falciparum mature gametocytes
11 It is the mature gametocytes of Plasmodium that are solely responsible for parasite 12 transmission from the mammalian host to the mosquito. They are therefore a logical target 13 for transmission blocking antimalarial interventions which aim to break the cycle of re14 infection and reduce the prevalence of malaria cases. Gametocytes however are not a 15 homogeneous cell population. They are...
متن کاملPromiscuous Expression of α-Tubulin II in Maturing Male and Female Plasmodium falciparum Gametocytes
BACKGROUND Antimalarial interventions designed to impact on the transmissible sexual stages of Plasmodium falciparum are evaluated by measurement of peripheral gametocyte carriage in vivo and infectivity to mosquitoes. Drug or vaccine-elicited effects may differentially affect the relative abundance of mature male and female sexual forms, and this can be measured by estimation of sex ratios bef...
متن کاملPromiscuous Expression of a-Tubulin II in Maturing Male and Female Plasmodium falciparum Gametocytes
Background: Antimalarial interventions designed to impact on the transmissible sexual stages of Plasmodium falciparum are evaluated by measurement of peripheral gametocyte carriage in vivo and infectivity to mosquitoes. Drug or vaccine-elicited effects may differentially affect the relative abundance of mature male and female sexual forms, and this can be measured by estimation of sex ratios be...
متن کاملDeveloping Gametocytes and Schizonts of Plasmodium Falciparum
The following is the description ol' plate XXII (figures 1 to 16) illustrating the article on Developing gametocytes and schizonts of Plasmodium falciparum ; A case showing all stages in the peripheral circulation', by B. M. Das Gupta and S. K. Ganguli, published in the October issue of the Gazette (p. 458) which was, by error, omitted. Figures 1 to 7 developing crescents, 6 and 7 showing two i...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Molecular and Biochemical Parasitology
سال: 2015
ISSN: 0166-6851
DOI: 10.1016/j.molbiopara.2015.03.006