Quantification of Listeria monocytogenes in fermented sausages by MPN-PCR method
نویسندگان
چکیده
منابع مشابه
Presence of Listeria monocytogenes in Mediterranean-Style Dry Fermented Sausages
The morphological, physiological and epidemiological features of L. monocytogenes, together with the severity of human listeriosis infections, make L. monocytogenes of particular concern for manufacturers of cold-stored "ready to eat" (RTE) foods. L. monocytogenes has been isolated from a wide variety of RTE foods and is responsible for several outbreaks associated with the consumption of RTE m...
متن کاملTHE EFFECTS OF pH, aW, AND LACTIC ACID BACTERIA ON LISTERIA MONOCYTOGENES IN FERMENTED SAUSAGES
The survival of inoculated in fermented sausages Listeria monocytogenes strain was studied. The sausages were prepared with and without starter cultures. The survival limits of L. monocytogenes and lactic acid bacteria (LAB) were determined as colony forming units per gram (cfu g) depending on water activity (aw) and pH on 0, 1 , 3, 5, 7, 14 and 21 days of maturation. The decreasing water activ...
متن کاملListeria monocytogenes serotype identification by PCR.
Serotyping is a universally accepted subtyping method for Listeria monocytogenes. Identification of the strain serotype permits differentiation between important food-borne strains (1/2a, 1/2b, and 4b) and provides a "gold standard" for comparing isolates analyzed in different labs and with different techniques. Although an efficient enzyme-linked immunosorbent assay serotyping protocol was des...
متن کاملA Fast, Reliable, and Sensitive Method for Detection and Quantification of Listeria monocytogenes and Escherichia coli O157:H7 in Ready-to-Eat Fresh-Cut Products by MPN-qPCR
In the present work we developed a MPN quantitative real-time PCR (MPN-qPCR) method for a fast and reliable detection and quantification of Listeria monocytogenes and Escherichia coli O157:H7 in minimally processed vegetables. In order to validate the proposed technique, the results were compared with conventional MPN followed by phenotypic and biochemical assays methods. When L. monocytogenes ...
متن کاملSensitive detection of viable Listeria monocytogenes by reverse transcription-PCR.
Detection of pathogens in contaminated food products by PCR can result in false-positive data due to the amplification of DNA from nonviable cells. A new method based on reverse transcription-PCR (RT-PCR) amplification of mRNA for the specific detection of viable Listeria monocytogenes was developed. The expression of three L. monocytogenes genes, iap, hly, and prfA, was examined to determine a...
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ژورنال
عنوان ژورنال: Letters in Applied Microbiology
سال: 2004
ISSN: 0266-8254,1365-2673
DOI: 10.1111/j.1472-765x.2004.01580.x