Quantitative fluorometric screening test for fecal porphyrins

Quantitative fluorometric screening test for fecal porphyrins.

We describe a fluorometric method for screening and quantifying porphyrins in stool. A small sample of stool is extracted with concentrated HCI and is diluted 200-fold in 3 mol/L HCI before analysis. An excitation scan is done from 350 to 450 nm, monitoring emission at 603 nm. Total porphyrin is estimated at the isosbestic point for coproporphyrin and protoporphyrin (402.5 nm). Monitoring emiss...

Quantitative Beutler test for newborn mass screening of galactosemia using a fluorometric microplate reader.

BACKGROUND The Beutler enzyme spot test is an effective assay for newborn mass screening of galactosemia, but it is qualitative and relies on visual interpretation. We describe a quantitative, instrumental modification of the assay. METHODS We modified the macroscopic visual Beutler enzyme spot test by adding extraction of blood components from filter paper, deproteinization with acetone-meth...

A semiquantitative screening test and quantitative assay for total fecal fat.

The gravimetric analysis of total fecal fat by a proposed chloroform extraction procedure has been shown to compare very closely with an accepted procedure involving a petroleum ether extraction, at all levelsof fecal fat. The decrease in the density of the chloroform by the fecal fat is used as a semiquantitative measure of fat concentration. This is accomplishedby noting the bouyancy of the c...

Fecal occult blood test screening for colorectal cancer.

In summary, high-quality scientific studies indicate that the use of the FOBT for colorectal cancer screening has a number of important advantages. The test is capable of detecting most early colorectal cancers and many advanced adenomas. It has been shown in randomized, controlled trials to reduce substantially colorectal cancer mortality and incidence. The FOBT is feasible, widely available, ...

Diet for fecal occult blood test screening: help or harm?