Quantitative separation of radioactive sterols and bile acids in human faeces

Quantitative Separation of Radioactive Sterols and Bile Acids in Human Faeces.

A method is described for the quantitative separation of radioactive bile acids and neutral sterols in human faeces on a potassium hydroxide-treated silicic acid column. Results obtained with faecal extracts containing metabolites derived from radioactive cholesterol, cholic acid, and chenodeoxycholic acid show that the efficiency of extraction is at least 96% and that mutual contamination betw...

Stero-Bile Acids and Bile Sterols

In a previous paper (XLVI of this series), the isolation of cyprinol and other three new bile sterols from the alkaline hydrolyzate of carp bile was reported. Also it was suggested that the chemical constitution of one of these new bile sterols would correspond to 3ƒ¿, 7ƒ¿, 12ƒ¿-trihydroxy-26, 27-epoxycholestane and the natural form of this new bile sterol might be 3ƒ¿,7ƒ¿,12ƒ¿,26,27-pentahydro...

Biliary bile acids, bile alcohols, and sterols of Alligator mississippiensis.

Bile from Alligator mississippiensis was found to contain a mixture of more than twenty bile acids, bile alcohols, and neutral sterols. Bile acids and bile alcohols were purified by reversed-phase high performance liquid chromatography and thin-layer chromatography. Concentrations were measured by gas-liquid chromatography on 1% HiEFF-8BP and identifications were made by mass spectrometry. The ...

Cxliii . the Distribution of Sterols in Human Faeces

Sterols 5. Bile Acids and Alcohols Structures, Occurrence and Biochemistry

The bile acids (mainly C24 but also C27) are the end products of cholesterol catabolism in animals, the main functions of which are to act as powerful detergents or emulsifying agents in the intestines to aid the digestion and absorption of fatty acids, monoacylglycerols and other fatty products and to prevent the precipitation of cholesterol in bile. Very many different bile acids and alcohols...