Rapid and simple G-quadruplex DNA aptasensor with guanine chemiluminescence detection
نویسندگان
چکیده
منابع مشابه
Ligands playing musical chairs with G-quadruplex DNA: a rapid and simple displacement assay for identifying selective G-quadruplex binders.
We report here the details of G4-FID (G-quadruplex fluorescent intercalator displacement), a simple method aiming at evaluating quadruplex-DNA binding affinity and quadruplex- over duplex-DNA selectivity of putative ligands. This assay is based on the loss of fluorescence upon displacement of thiazole orange from quadruplex- and duplex-DNA matrices. The original protocol was tested using variou...
متن کاملReduction-Responsive Guanine Incorporated into G-Quadruplex-Forming DNA.
Stimulus-responsive biomolecules are attractive targets to understand biomolecule behaviour as well as to explore their therapeutic and diagnostic applications. We demonstrate that a reduction-responsive cleavable group (chemically caged unit) introduced into the guanine ring enables modulation of the secondary structure transition of an oligonucleotide in a reduction-responsive and traceless m...
متن کاملA G-quadruplex-based Label-free Fluorometric Aptasensor for Adenosine Triphosphate Detection.
A G-quadruplex-based, label-free fluorescence assay was demonstrated for the detection of adenosine triphosphate (ATP). A double-stranded DNA (dsDNA), hybridized by ATP-aptamer and its complementary sequence, was employed as a substrate for ATP binding. SYBR Green I (SG I) was a fluorescent probe and exonuclease III (Exo III) was a nuclease to digest the dsDNA. Consequently, in the absence of A...
متن کاملHuman Pif1 helicase is a G-quadruplex DNA-binding protein with G-quadruplex DNA-unwinding activity.
Pif1 proteins are helicases that in yeast are implicated in the maintenance of genome stability. One activity of Saccharomyces cerevisiae Pif1 is to stabilize DNA sequences that could otherwise form deleterious G4 (G-quadruplex) structures by acting as a G4 resolvase. The present study shows that human Pif1 (hPif1, nuclear form) is a G4 DNA-binding and resolvase protein and that these activitie...
متن کاملPotassium-sensitive G-quadruplex DNA for sensitive visible potassium detection.
A novel visual method for K(+) detection is developed utilizing a K(+)-sensitive G-quadruplex DNA named PS5.M as the sensing element. In the absence of K(+), PS5.M is in a random coil state and does not bind hemin. Upon addition of K(+), PS5.M folds into the quadruplex structure stabilized by K(+). Such quadruplex structure is able to bind hemin to form the hemin-G-quadruplex DNAzyme that catal...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biosensors and Bioelectronics
سال: 2014
ISSN: 0956-5663
DOI: 10.1016/j.bios.2013.09.017