Rapid Detection of the Fibrinogen Aα16Arg→His Mutation

Rapid nonradioactive detection of the major cystic fibrosis mutation.

Comparison of rapid clottable fibrinogen assays.

Measurements of fibrinogen concentration by use of two rapid methods (clot.weight method and th rombin-turbidity method) were compared with the reference thrombin.coagulation method of Ratnoff and Menzie [J. Lab. Clin. Med. 37, 316 (1951)]. Both rapid methods gave results (in the high, middle, and low ranges) that correlated well with those of the thrombin-coagulation method. However, the throm...

CCR: a rapid and simple approach for mutation detection.

We describe a simple approach for detecting known mutations in genomic DNA. The strategy entails a DNA amplification reaction that combines the use of thermostable DNA polymerase and ligase, and that has been designated the Combined Chain Reaction (CCR). CCR consists of four phases: denaturation, annealing, elongation and ligation. Unlike most PCR-based mutation detection systems it relies on m...

Contrast Enhancement of Mammograms for Rapid Detection of Microcalcification Clusters

Introduction Breast cancer is one of the most common types of cancer among women.  Early detection of breast cancer is the key to reducing the associated mortality rate. The presence of microcalcifications clusters (MCCs) is one of the earliest signs of breast cancer. Due to poor imaging contrast of mammograms and noise contamination, radiologists may overlook some diagnostic signs, specially t...

A rapid, early detection of oral squamous cell carcinoma: Real time PCR based detection of tetranectin

The current study is focused on determining the mRNA expression levels of tetranectin, to detect oral squamous cell carcinoma (OSCC) and thus aiding in its classification at an early stage. RNA was isolated and cDNA synthesis was performed from the saliva samples of the patients and healthy individuals. A semiquantitative PCR based analysis was performed prior to quantitative and expression bas...