Rapid procedure for isolation of plasmid DNA and application to epidemiological analysis
نویسندگان
چکیده
منابع مشابه
A rapid procedure for the isolation of plasmid DNA from environmental bacteria.
The INSTA-MINI-PREP method, a rapid protocol for plasmid DNA extraction, was originally developed to prepare plasmid DNA from 1 to 3 ml miniprep Escherichia coli cultures. Direct extraction of plasmid DNA is achieved by a two-phase solution which is separated by centrifugation in the presence of the INSTA-PREP gel barrier material. This method has been successfully tested on various environment...
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Isolation of highly purified plasmid DNA from E. coli is an essential step in many techniques of current molecular biology. Traditional techniques for plasmid isolation are the alkaline lysis technique of Birnboim and Doly (2) and the boiling method of Holmes and Quigley (4), which are normally followed by a further purification by cesium chloride gradient centrifugation (9). Though the purity ...
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A procedure for extracting plasmid DNA from bacterial cells is described. The method is simple enough to permit the analysis by gel electrophoresis of 100 or more clones per day yet yields plasmid DNA which is pure enough to be digestible by restriction enzymes. The principle of the method is selective alkaline denaturation of high molecular weight chromosomal DNA while covalently closed circul...
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Rapid plasmid DNA isolation from mucoid gram-negative bacteria.
Exopolysaccharides interfere with the isolation and characterization of plasmid DNA from gram-negative bacteria. To repress capsular polysaccharide production, bacteria were cultured in medium containing bismuth nitrate and sodium salicylate. Rapid removal of other contaminating bacterial surface components was achieved by mild acidic zwitterionic detergent extraction. After treatment, bacteria...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1984
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.20.4.608-613.1984