Rapid screening of microsatellite markers for polymorphisms using SYBR®Green I and a DNA sequencer
نویسندگان
چکیده
منابع مشابه
Rapid screening of microsatellite markers for polymorphisms using SYBR green I and a DNA sequencer.
Vol. 36, No. 3 (2004) BioTechniques 409 The process of microsatellite development and profiling involves three primary steps: (i) isolating regions of genomic DNA that contain microsatellite loci; (ii) developing strategies for screening each locus, which requires designing primers for amplification, optimizing reaction conditions, and screening for variation; and (iii) genotyping sampled indiv...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2004
ISSN: 0736-6205,1940-9818
DOI: 10.2144/04363bm06