Rapid West Nile Virus Antigen Detection
نویسندگان
چکیده
منابع مشابه
Rapid West Nile Virus Antigen Detection
We compared the VecTest WNV antigen assay with standard methods of West Nile virus (WNV) detection in swabs from American Crows (Corvus brachyrhynchos) and House Sparrows (Passer domesticus). The VecTest detected WNV more frequently than the plaque assay and was comparable to a TaqMan reverse transcription-polymerase chain reaction.
متن کاملWest Nile Virus Detection and Commercial Assays
1154 Emerging Infectious Diseases • www.cdc.gov/eid • Vol. 11, No. 7, July 2005 PanBio Pty. Ltd., Brisbane, Australia). The same acute-phase serum was tested for flavivirus RNA by seminested reverse transcription–polymerase chain reaction (RT-PCR) by using flavivirus consensus primers PF1S and PF2R as previously described (1) in conjunction with the sense primer PF3S (GCIATHTGGTAYATGTGGYT). Att...
متن کاملWest Nile Virus Detection in American Crows
A dipstick immunochromatographic assay used for West Nile virus (WNV) detection in mosquitoes was investigated for application to testing of fecal, saliva, and tissue samples from dead American Crows (Corvus brachyrhynchos). Results suggest that VecTest may be an efficient method for WNV detection in field-collected, dead American Crows, although confirmation of results and further investigatio...
متن کاملWest Nile Virus Detection in Urine
We report West Nile virus (WNV) RNA in urine collected from a patient with encephalitis 8 days after symptom onset. Viral RNA was detected by reverse transcriptase-polymerase chain reaction (RT-PCR). Sequence and phylogenetic analysis confirmed the PCR product to have > or = 99% similarity to the WNV strain NY 2000-crow3356.
متن کاملRapid Antigen-Capture Assay To Detect West Nile Virus in Dead Corvids
The utility of the VecTest antigen-capture assay to detect West Nile virus (WNV) in field-collected dead corvids was evaluated in Manitoba and Ontario, Canada, in 2001 and 2002. Swabs were taken from the oropharynx, cloaca, or both of 109 American Crows, 31 Blue Jays, 6 Common Ravens, and 4 Black-billed Magpies from Manitoba, and 255 American Crows and 28 Blue Jays from Ontario. The sensitivity...
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ژورنال
عنوان ژورنال: Emerging Infectious Diseases
سال: 2005
ISSN: 1080-6040,1080-6059
DOI: 10.3201/eid1110.040394