Real-Time PCR-Based Method for Assaying the Purity of Bacterial Artificial Chromosome Preparations
نویسندگان
چکیده
منابع مشابه
Real-time PCR-based method for assaying the purity of bacterial artificial chromosome preparations.
Large-scale genomic sequencing projects (such as that being undertaken for the human genome project) often prepare large insert (100–200 kb) libraries in bacterial artificial chromosome (BAC) vectors. Short insert libraries are then constructed from individual isolated BACs, and clones from the short insert libraries are randomly selected for sequencing to the requisite coverage. BAC DNA is fir...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2000
ISSN: 0736-6205,1940-9818
DOI: 10.2144/00293bm01