Real-time PCR optimization to identify environmental Vibrio spp. strains
نویسندگان
چکیده
منابع مشابه
Real-time PCR optimization to identify environmental Vibrio spp. strains.
AIMS To identify Vibrio vulnificus, Vibrio cholerae and Vibrio alginolyticus using standardized DNA extraction method and real-time PCR assays, among a large number of bacterial strains isolated from marine environment. METHODS AND RESULTS Methods for DNA extraction and real-time PCR were standardized to identify a large number of Vibrio spp. strains isolated through regular collection campai...
متن کاملDetermination of ctxAB expression in Vibrio cholerae Classical and El Tor strains using Real-Time PCR
Cholera is an infection of the small intestines caused by the bacterium V. cholerae. It is a major cause of health threat and also a major cause of death worldwide and especially in developing countries. The major virulence factor produced by V. cholerae during infection is the cholera toxin. Total mRNA extraction and reverse transcription was performed for making ctxAB cDNA. Relative Real-Time...
متن کاملdetermination of ctxab expression in vibrio cholerae classical and el tor strains using real-time pcr
cholera is an infection of the small intestines caused by the bacterium v. cholerae. it is a major cause of health threat and also a major cause of death worldwide and especially in developing countries. the major virulence factor produced by v. cholerae during infection is the cholera toxin. total mrna extraction and reverse transcription was performed for making ctxab cdna. relative real-time...
متن کاملDetermination of ctxAB expression in Vibrio cholerae Classical and El Tor strains using Real-Time PCR
Cholera is an infection of the small intestines caused by the bacterium V. cholerae. It is a major cause of health threat and also a major cause of death worldwide and especially in developing countries. The major virulence factor produced by V. cholerae during infection is the cholera toxin. Total mRNA extraction and reverse transcription was performed for making ctxAB cDNA. Relative Real-Time...
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A molecular typing study on Vibrio strains implicated in shrimp disease outbreaks in New Caledonia and Japan was conducted by using AP-PCR (arbitrarily primed PCR). It allowed rapid identification of isolates at the genospecies level and studies of infraspecific population structures of epidemiological interest. Clusters identified within the species Vibrio penaeicida were related to their area...
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ژورنال
عنوان ژورنال: Journal of Applied Microbiology
سال: 2012
ISSN: 1364-5072
DOI: 10.1111/j.1365-2672.2012.05350.x