Real-time PCR (qPCR) primer design using free online software
نویسندگان
چکیده
منابع مشابه
qPCR primer design revisited
Primers are arguably the single most critical components of any PCR assay, as their properties control the exquisite specificity and sensitivity that make this method uniquely powerful. Consequently, poor design combined with failure to optimise reaction conditions is likely to result in reduced technical precision and false positive or negative detection of amplification targets. Despite the f...
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ژورنال
عنوان ژورنال: Biochemistry and Molecular Biology Education
سال: 2011
ISSN: 1470-8175
DOI: 10.1002/bmb.20461