Recombinant cDNA Probe from Bluetongue Virus Genome Segment 10 for Identification of Bluetongue Virus
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منابع مشابه
Bluetongue virus tubules made in insect cells by recombinant baculoviruses: expression of the NS1 gene of bluetongue virus serotype 10.
Bluetongue virus (BTV) forms tubules in mammalian cells. These tubules appear to be composed of only one type of protein, NS1, a major nonstructural protein of the virus. To obtain direct evidence for the origin of the tubules, the complete M6 gene of BTV serotype 10 was inserted into the baculovirus transfer vector pAcYM1, so that it was under the control of the polyhedrin promoter of Autograp...
متن کاملCharacterization of a second open reading frame in genome segment 10 of bluetongue virus
Viruses have often evolved overlapping reading frames in order to maximize their coding capacity. Until recently, the segmented dsRNA genome of viruses of the Orbivirus genus was thought to be monocistronic, but the identification of the bluetongue virus (BTV) NS4 protein changed this assumption. A small ORF in segment 10, overlapping the NS3 ORF in the +1 position, is maintained in more than 3...
متن کاملInterferon induction by bluetongue virus and bluetongue virus ribonucleic acid.
EKSTEEN, P. A . L. & HUISMAN S, H. Interferon induction by bluetongue virus and bluetongue virus ribonucleic acid. Onderstep oort]. vet . R es. 39(3), 125-132 (1972). The stimulation of interferon synthesis by bluetongue virus and by bluetongue virus ribonucleic acid was investigated in order to determine if there is a difference in the mechanism of induction. The molecular mass of the interfer...
متن کاملThe complete nucleotide sequence of genome segment 7 of bluetongue virus, serotype 1 from South Africa.
The complete sequence of genome segment 7, of bluetongue was 98.3% identity and 98.9% similarity (including conservative virus, serotype 1 South Africa (BTV1SA), which encodes the changes). serogroup-specific antigen, VP7 (1), was determined from a full length cDNA clone synthesized using terminal oligonucleotides REFERENCES as primers (2). Genome segment 7 from BTV1SA is the same length as the...
متن کاملMolecular analysis of genome segment-3 of bluetongue virus serotype 12 isolates from Haryana
Aim The present study was designed to characterize the genome segment 3 (Seg-3) of bluetongue virus (BTV) serotype 12 isolates from different outbreaks of Bluetongue disease in Haryana, India. Materials and Methods Blood and swab samples were collected from goat and sheep suspected to be suffering of BT from different outbreaks from Gurugram, Sirsa, Hisar, and Karnal districts of Haryana. The...
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ژورنال
عنوان ژورنال: Journal of Veterinary Diagnostic Investigation
سال: 1989
ISSN: 1040-6387,1943-4936
DOI: 10.1177/104063878900100308