Recombinant expression of the rhoptry protein Clag3.1 as a green fluorescent chimeric protein in Plasmodium falciparum
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چکیده
منابع مشابه
Trafficking of Plasmodium falciparum chimeric rhoptry protein with Brefeldin A.
Trafficking of the rhoptry chimeric protein RhopH2-GFP, which contains RhopH2 signal peptide plus the downstream five amino acids, was dissected by treating parasites with Brefeldin A at three different time points. Twenty eight hrs-stage trophozoites accumulated the chimera within the parasite endoplasmic reticulum. In 32 hrs-stage schizonts, the chimera was distributed in the parasite cytopla...
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The most deadly Apicomplexan parasite is Plasmodium falciparum because it causes the globally spread human malaria especially in Africa. Like other members of Apicomplexa, Plasmodium is an intracellular parasite that uses an elaborate complex of apical secretory organelles (rhoptries, micronemes and dense granules) to invade its host cells and immediately accommodate itself in a parasitophorous...
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Invasive forms of apicomplexan parasites contain secretory organelles called rhoptries that are essential for entry into host cells. We present a detailed characterization of an unusual rhoptry protein of the human malaria parasite Plasmodium falciparum, the rhoptry-associated membrane antigen (RAMA) that appears to have roles in both rhoptry biogenesis and host cell invasion. RAMA is synthesiz...
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The aim of this study was to construct, expression of a novel recombinant chimeric protein consisting of Pyruvate dehydrogenase beta subunit (PDHB) and high antigenic region of integral membrane lipoprotein P80 of Mycoplasma agalactiae as a potential diagnostic tool. The full-length sequence of pdhb and a portion of antigenic regions of P80 were selected and analyzed by CLC ma...
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Rhoptry-associated protein 1 (RAP1) of Plasmodium falciparum is a potential component of a malaria vaccine. We have expressed in Escherichia coli eight recombinant RAP1 proteins representing almost the entire sequence of the mature protein and assessed the antigenicity of the proteins by immunization of mice. Antisera to six of the recombinant proteins reacted specifically with parasite-derived...
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ژورنال
عنوان ژورنال: Delta Journal of Science
سال: 2013
ISSN: 2735-5306
DOI: 10.21608/djs.2013.139775