Recombinational-type transfer of viral DNA during bacteriophage 2C replication in Bacillus subtilis
نویسندگان
چکیده
منابع مشابه
DNA of Bacillus subtilis bacteriophage SPP1: physical mapping and localization of the origin of replication.
The genome of Bacillus subtilis bacteriophage SPP1, a linear, 28.5-megadalton DNA duplex, was mapped by analysis with the restriction endonucleases endo R.Sal I, Sma I, Xba I, Bgl I, Bgl II, and EcoRI. The SPP1 genome, like that of the Salmonella typhimurium phage, P22, was found to be a terminally repetitious, circularly permuted molecule. 6-(p-Hydroxyphenylazo)uracil, a selective, reversible ...
متن کاملRevised restriction maps of Bacillus subtilis bacteriophage phi 105 DNA.
Physical mapping of Bacillus subtilis temperate phage phi 105 DNA was carried out by using restriction endonucleases EcoRI, SmaI, and KpnI, and a new revised EcoRI cleavage map is presented. In addition, the EcoRI cleavage maps of six specialized transducing phages carrying sporulation genes of B. subtilis were revised.
متن کاملTargeted isolation of a designated region of the Bacillus subtilis genome by recombinational transfer.
A method for positional cloning of the Bacillus subtilis genome was developed. The method requires a set of two small DNA fragments that flank the region to be copied. A 38-kb segment that carries genes ppsABCDE encoding five enzymes for antibiotic plipastatin synthesis and another genome locus as large as 100 kb including one essential gene were examined for positional cloning. The positional ...
متن کاملTransduction in Bacillus subtilis by bacteriophage SPP1.
Lysates of the virulent bacteriophage SPP1 were shown to be capable of mediating generalized transduction. Suppressible mutants of this bacteriophage (sus) were capable of transduction at a lower multiplicity of infection than virulent SPP1. Linkage analysis demonstrated that bacteriophage SPP1 transduced segments of the genome equal in size to that transferred by SP10. This bacteriophage shoul...
متن کاملRegion dependent efficiency for recombinational transfer of the Bacillus subtilis 168 genome.
Submega-sized regions of the Bacillus subtilis genome were cloned to plasmid by the B. subtilis Recombinational Transfer (BReT) method. BReT efficiency depends not only on the genome location but also on the choice of sequences for simultaneous homologous recombination during BReT. In an extreme case, a 91-kb region that was unsuccessful on the first attempt was obtained when the slightly shift...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1976
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.17.3.718-726.1976