Redox Mechanisms of Cytoprotection by Bcl-2

Mechanisms of action of Bcl-2 family proteins.

The Bcl-2 family of proteins controls a critical step in commitment to apoptosis by regulating permeabilization of the mitochondrial outer membrane (MOM). The family is divided into three classes: multiregion proapoptotic proteins that directly permeabilize the MOM; BH3 proteins that directly or indirectly activate the pore-forming class members; and the antiapoptotic proteins that inhibit this...

Gastrointestinal cytoprotection by prostaglandins.

Prostaglandins (PGs) are widely distributed throughout the gastrointestinal tract and affect a variety of gastrointestinal functions. The observation that PGs of the E and A series are potent inhibitors of gastric acid secretion and that PGs, especially the E compounds, are released into the gastric lumen during vagal or gastrin stimulation suggests a possible physiological role for these agent...

Role of caspase 3-dependent Bcl-2 cleavage in potentiation of apoptosis by Bcl-2.

Previous studies from our laboratory have demonstrated that Bcl-2 has a proapoptotic effect on neocarzinostatin (NCS)-treated PC12 pheochromocytoma cells. In the present study, we examine the mechanisms of this effect and demonstrate its relevance for the in vivo situation. Four hours after NCS treatment, a 23-kDa cleavage product of Bcl-2 was detected in whole cell lysates of bcl-2-transfected...

Mechanisms of Action of Bcl-2 Family Proteins Subject Collection Cell Survival and Cell Death Mechanisms of Action of Bcl-2 Family Proteins

Bcl-2-related protein A1 is an endogenous and cytokine-stimulated mediator of cytoprotection in hyperoxic acute lung injury.

Hyperoxic acute lung injury (HALI) is characterized by a cell death response with features of apoptosis and necrosis that is inhibited by IL-11 and other interventions. We hypothesized that Bfl-1/A1, an antiapoptotic Bcl-2 protein, is a critical regulator of HALI and a mediator of IL-11-induced cytoprotection. To test this, we characterized the expression of A1 and the oxygen susceptibility of ...