Regulation of Metaphase by a Maturation-Promoting Factor. (meiosis/mitosis/cell cycle/metaphase/maturation-promoting factor)
نویسندگان
چکیده
منابع مشابه
Maturation promoting factor and cell cycle regulation.
Cell cycles in early amphibian embryos are characterized by the absence of G1 and G2 phases. The simple cycle of S phase and mitosis does show similarities with other systems, particularly in the presence of cytoplasmic components advancing nuclei into DNA synthesis and mitosis. Maturation-promoting factor induces nuclear envelope breakdown and subsequent chromosome condensation. Cytoplasmic fa...
متن کاملMaturation-promoting factor and the regulation of the cell cycle.
Maturation-promoting factor (MPF) is a cell cycle control element able to cause metaphase when injected into amphibian oocytes or when incubated with nuclei in a cell-free system. Highly purified MPF consists of a complex between a 34K (K = 10(3) Mr) serine/threonine protein kinase, identified as a Xenopus homolog of the cdc2+ gene product, p34cdc2, and a 45K substrate, identified as a Xenopus ...
متن کاملPreliminary characterization of maturation-promoting factor from yeast Saccharomyces cerevisiae.
It has been known for some time that maturation-promoting factor (MPF) appears in a wide variety of eukaryotic cells at M phase and exerts equal M-phase-promoting activity in both meiotic cells and mitotic cells in a non-specific manner. MPF was extracted from cdc20 mutant cells of the yeast Saccharomyces cerevisiae synchronized at M phase by incubation at the restrictive temperature. When inje...
متن کاملRegulation of Xenopus p21-activated kinase (X-PAK2) by Cdc42 and maturation-promoting factor controls Xenopus oocyte maturation.
Signal transduction cascades involved in regulation of the cell cycle machinery are poorly understood. In the Xenopus oocyte model, meiotic maturation is triggered by MPF, a complex of p34(cdc2)-cyclin B, which is activated in response to a progesterone signal by largely unknown mechanisms. We have previously shown that the p21-activated kinase (PAK) family negatively regulates the MPF amplific...
متن کاملActive maturation-promoting factor is present in mature mouse oocytes
Cytoplasmic extracts of meiotically mature mouse oocytes were injected into immature Xenopus laevis oocytes, which underwent germinal vesicle breakdown within 2 h. Germinal vesicle breakdown was not inhibited by incubation of the Xenopus oocytes in cycloheximide (20 micrograms/ml). Identically prepared extracts of meiotically immature mouse oocytes, arrested at the germinal vesicle stage by dib...
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ژورنال
عنوان ژورنال: Development, Growth and Differentiation
سال: 1988
ISSN: 0012-1592,1440-169X
DOI: 10.1111/j.1440-169x.1988.00105.x