Regulation of Nitrogenase Gene Expression by Transcript Stability in the Cyanobacterium Anabaena variabilis
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چکیده
منابع مشابه
Regulation of nitrogenase gene expression by transcript stability in the cyanobacterium Anabaena variabilis.
The nitrogenase gene cluster in cyanobacteria has been thought to comprise multiple operons; however, in Anabaena variabilis, the promoter for the first gene in the cluster, nifB1, appeared to be the primary promoter for the entire nif cluster. The structural genes nifHDK1 were the most abundant transcripts; however, their abundance was not controlled by an independent nifH1 promoter, but rathe...
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The filamentous cyanobacterium Anabaena variabilis ATCC 29413 fixes nitrogen under aerobic conditions in specialized cells called heterocysts that form in response to an environmental deficiency in combined nitrogen. Nitrogen fixation is mediated by the enzyme nitrogenase, which is very sensitive to oxygen. Heterocysts are microxic cells that allow nitrogenase to function in a filament comprise...
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Little is known about the regulation of nitrogenase genes in cyanobacteria. Transcription of the nifH1 and vnfH genes, encoding dinitrogenase reductases for the heterocyst-specific Mo-nitrogenase and the alternative V-nitrogenase, respectively, was studied by using a lacZ reporter. Despite evidence for a transcription start site just upstream of nifH1 and vnfH, promoter fragments that included ...
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Physiological experiments indicate that Anabaena variabilis can express either a Vor a Fe-nitrogenase in addition to the conventional, Mo-containing enzyme complex. The occur rence o f the Fe-nitrogenase in A. variabilis can also be concluded from D N A -D N A hybridiza tion experiments using cloned anfW or nifW probes coding for the smaller subunit (= nitroge nase reductase) o f the Fe-nitr...
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A cell-free system exhibiting both photophosphorylation (P /2e = 1) and oxidative phosphoryla tion (P/O up to 0 .8 ) is described for the cyanobacterium Anabaena variabilis. NADH and N A D PH were found to be equally effective as electron donors for oxidative phosphorylation. Low concentrations of U H D B T , an inhibitor of the cytochrome blc complex of mitochondria and chloroplasts. were fou...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 2014
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.02045-14