Regulatory effects of laminin derived peptide on microtissue formation for tissue engineered scaffold-free constructs

Effect of seeding technique and scaffold material on bone formation in tissue-engineered constructs.

The aim of the present study was to test the hypothesis that both scaffold material and the type of cell culturing contribute to the results of in vivo osteogenesis in tissue-engineered constructs in an interactive manner. CaCO3 scaffolds and mineralized collagen scaffolds were seeded with human trabecular bone cells at a density of 5 x 10(6) cells/cm(3) and were left to attach under standard c...

Cytotoxicity of Chitosan Derived from Shrimp for Bone Scaffold on Adipose Tissue-Derived Mesenchymal Stem Cells

Bioengineering Human Scaffold-free Cartilage Constructs Using Paediatric Auricular Tissue

Currently, patients with external ear deformities rely on hyaline cartilage grafts or silicone prosthesis to provide them with adequate external ear reconstruction. Scaffold-free auricular tissue engineering provides a means of creating patient specific cartilage constructs solely using their own cells, ensuring biocompatibility, long term stability, and reduced morbidity. Here, we have develop...

Adipose Tissue Engineering: Comparing Scaffold-free and Fibrin-based Constructs

Introduction Success of adipose tissue engineering for soft tissue repair has been limited by poor cell survival and insufficient adipogenic differentiation. Although the choice of scaffold can influence these parameters, there is a limited number of studies focussing on the role of scaffolds in cell survival and. adipogenic differentiation. In this study we examined how three-dimensional scaff...

*The Effects of Scaffold Remnants in Decellularized Tissue-Engineered Cardiovascular Constructs on the Recruitment of Blood Cells

Decellularized tissue-engineered heart valves (DTEHVs) showed remarkable results in translational animal models, leading to recellularization within hours after implantation. This is crucial to enable tissue remodeling. To investigate if the presence of scaffold remnants before implantation is responsible for the fast recellularization of DTEHVs, an in vitro mesofluidic system was used. Human g...