Relationship between morphological change and superoxide generation in rat peritoneal mast cells.
نویسندگان
چکیده
منابع مشابه
Relationship between arachidonate generation and exocytosis in permeabilized mast cells.
Using rat mast cells permeabilized with streptolysin O we show that release of arachidonate generally occurs under similar but not identical conditions to those that cause exocytosis of beta-N-acetylglucosaminidase (hexosaminidase). Thus, hexosaminidase secretion and arachidonate release both require provision of Ca2+ together with a guanine nucleotide but exocytosis occurs at lower concentrati...
متن کاملGlycolysis in Rat Peritoneal Mast Cells
Glycolytic activity of rat peritoneal mast cells has been measured by the Cartesian ampulla diver technique. The rates of anaerobic glycolysis, expressed as CO(2) expelled from a bicarbonate medium, are 1.70 x 10(-6) microl and 1.43 x 10(-6) microl per cell per hour with and without glucose, respectively. The aerobic glycolysis rate in the presence of glucose, assuming the respiratory quotient ...
متن کاملNative heparin from rat peritoneal mast cells.
[35S]Heparin was produced in vitro by incubation of rat peritoneal mast cells with [35S]sulfate and in vivo by injection of [35S]sulfate into rats. The [35S]heparin together with nonlabeled heparin in the mast cells was isolated in native form by mild methods that avoided the use of proteolytic enzymes or high alkali concentrations. The heparin had low anticoagulant activity. Incubations of mas...
متن کاملRespiration of Rat Peritoneal Mast Cells
Methods for microgasometry of a few hundred mast cells are described. The Cartesian ampulla diver technique is used. The sample size is determined by counting the cells within the diver. The respiration rates at 37 degrees C, expressed in microliters per cell per hour, are 0.29 x 10(-6) without substrate and 0.47 x 10(-6) with glucose.
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ژورنال
عنوان ژورنال: Japanese Journal of Pharmacology
سال: 1996
ISSN: 0021-5198
DOI: 10.1016/s0021-5198(19)37093-3