Reporter gene insertions reveal a strictly B lymphoid-specific expression pattern of Pax5 in support of its B cell identity function.

Reporter gene insertions reveal a strictly B lymphoid-specific expression pattern of Pax5 in support of its B cell identity function.

The transcription factor Pax5 is essential for B cell commitment and development. Although the detail Pax5 expression pattern within the hemopoietic system is still largely unknown, we previously reported that Pax5 is monoallelically transcribed in pro-B and mature B cells. In this study, we have investigated the expression of Pax5 at single-cell resolution by inserting a GFP or human Cd2 indic...

CORRECTIONS Reporter Gene Insertions Reveal a Strictly B Lymphoid-Specific Expression Pattern of Pax5 in Support of Its B Cell Identity Function

Pax5 maintains cellular identity by repressing gene expression throughout B cell differentiation.

The transcription factor Pax5 is required for many aspects of B-lymphopoiesis including lineage commitment, immunoglobulin rearrangement, pre-BCR signalling and mature B cell survival. Pax5 regulates B cell lineage commitment by concurrently activating cell specific gene expression as well as suppressing the expression of genes associated with non-B cell fates. The identity of the molecular tar...

The B-cell identity factor Pax5 regulates distinct transcriptional programmes in early and late B lymphopoiesis.

Pax5 controls the identity and development of B cells by repressing lineage-inappropriate genes and activating B-cell-specific genes. Here, we used genome-wide approaches to identify Pax5 target genes in pro-B and mature B cells. In these cell types, Pax5 bound to 40% of the cis-regulatory elements defined by mapping DNase I hypersensitive (DHS) sites, transcription start sites and histone modi...

The B cell identity factor Pax5 regulates distinct transcriptional programs in early and late B lymphopoiesis

As you can see, the referees appreciate that the analysis provides new insight into Pax5-mediate gene regulation during B cell development and are overall supportive of publication here. They raise a number of specific concerns that shouldn't involve too much additional work to address. What is also needed is a better understanding of how Pax5 binds distinct targets in pro-B and mature B cells....