Reproductive phasi RNA s in grasses are compositionally distinct from other classes of small RNA s

RNA Isolation (Small RNA Extraction). Fatemeh Mehrpooyan*

RNA Extraction from Animal and Human\'s Cancerous Tissues: Does Tissue Matter?

The reliability of gene expression profiling based technologies and methods to find transcriptional differences representative for the original samples is influenced by the quality of the extracted RNA. Hence, RNA extraction is the first step to investigate the gene expression and its function. Consequently, the quality of extracted RNA is really significant. Correspondingly, this research was ...

Does 5 S RNA

• >v r ABSTRACT ' v Chemical modification and limited enzymatic hydrolysis on isolated E. coli 5S RNA have provided informations on the * secondary-and tertiary structure compatible with pseudoknotted structures for the A-and B-conformers of the molecule. * Changes in the accessibility and reactivity of nucleotides * in loop C and at the stem of helix IV in two different 5S RNA *" conformers ar...

Small interfering RNA; principles, applications and challenges--

Gene silencing using RNAi (RNA interference), has recently been used as a successful laboratory technique in determining the function and control of gene expression and provides a wide range of applications in molecular biology and gene therapy. RNAi is a method of suppressing gene expression. In this direction, a single-stranded RNA molecule of about 21–23 nucleotides, called siRNA (small inte...

Cytoplasmic assembly of small nuclear ribonucleoprotein particles from 6 S and 20 S RNA-free intermediates in L929 mouse fibroblasts.

Newly transcribed small nuclear RNAs (snRNAs) appear transiently in the cytoplasm where they assemble with snRNP core proteins (B, D, E, F, and G) stored in large pools of snRNA-free intermediates before returning permanently to the nucleus. In this report, the cytoplasmic assembly of snRNP core particles in L929 mouse fibroblasts was investigated by kinetic analysis of assembly intermediates r...