Reprogramming of Round Spermatids by the Germinal Vesicle Cytoplasm in Mice
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چکیده
منابع مشابه
Reprogramming of Round Spermatids by the Germinal Vesicle Cytoplasm in Mice
The birthrate following round spermatid injection (ROSI) remains low in current and evidence suggests that factors in the germinal vesicle (GV) cytoplasm and certain substances in the GV such as the nucleolus might be responsible for genomic reprogramming and embryonic development. However, little is known whether the reprogramming factors in GV oocyte cytoplasm and/or nucleolus in GV are benef...
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Epigenetic reprogramming in somatic cells induced by extract from germinal vesicle stage pig oocytes.
Genomic reprogramming factors in the cytoplasm of germinal vesicle (GV) stage oocytes have been shown to improve the efficiency of producing cloned mouse offspring through the exposure of nuclei to a GV cytoplasmic extract prior to somatic cell nuclear transfer (SCNT) to enucleated oocytes. Here, we developed an extract of GV stage pig oocytes (GVcyto-extract) to investigate epigenetic reprogra...
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Background: The efficiency of in vitro maturation (IVM) has remained low despite extensive effort to understand the culture conditions. This study investigated the effect of cumulus cells and meiotic inhibitors on oocyte developmental competence in the presence of Alpha Lipoic acid (ALA) Materials and Methods: Mouse germinal vesicle oocytes were considered in cumulus-denuded oocytes (CDOs) and ...
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ژورنال
عنوان ژورنال: PLoS ONE
سال: 2013
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0078437