Resolution of a long lived fluorescence component from D1/D2/ cytochrome b -559 reaction centres
نویسندگان
چکیده
منابع مشابه
Cytochrome b-559 and proton conductance in oxygenic photosynthesis.
Although cytochrome b-559 has long been known as a membrane-bound redox component closely linked to the reaction center of the oxygen-generating photosystem (PSII), its role in photosynthesis has remained obscure. This paper reports evidence and outlines a hypothesis in support of a "b-559 cycle"-i.e., a light-induced, cytochrome b-559-dependent, cyclic electron transport pathway around PSII th...
متن کاملISOLATION AND CHARACTERIZATION OF CYTOCHROME b-559 FROM CHLOROPLASTS AND ETIOPLASTS OF BARLEY
A procedure permitting the isolation of large amounts of cytochrome b-559 from chloroplasts of barley is described. The isolated cytochrome b-559 has an apparent molecular weight of 120,000. The protein equivalent weight per mole of heme is 30,000. Depending on the electrophoretic conditions used, this molecule can be dissociated into components with apparent molecular weights of 31,000, 30,000...
متن کاملPhotosynthetic water oxidation in cytochrome b(559) mutants containing a disrupted heme-binding pocket.
The role of cytochrome b(559) in photosynthetic oxygen evolution has been investigated in three chloroplast mutants of Chlamydomonas reinhardtii, in which one of the two histidine axial ligands to the heme, provided by the alpha subunit, has been replaced by the residues methionine, tyrosine, and glutamine. Photosystem two complexes functional for oxygen evolution could be assembled in the meth...
متن کاملEvidence for a hetero-oligomeric structure of the chloroplast cytochrome b-559
A second nonhomologous polypeptide in the thyiakoid membrane cytochrome b-559 has been suggested by the finding of a smaller reading frame just slightly downstream from that corresponding to the 9 kDa cytochrome polypeptide that is dominant on a Coomassie-stained gel. This reading frame encoded a 39residue polypeptide that was similar in having a central hydrophobic domain of 25-26 residues and...
متن کاملChlorophyll and cytochrome 6-559 content of the photochemical reaction center of photosystem II
Photosystem II reaction centers comprised of the Dl, D2 and cytochrome b-559 polypeptides were isolated from welldefined oxygen-evolving reaction center preparations using either a combination of LiClO, and dodecyhnaltoside, or Triton X-100 alone. Yields of chlorophyll and cytochrome b-559 for both preparative methods were compared, and pigment contents were compared based on 2.0 b-559 per reac...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1989
ISSN: 0014-5793
DOI: 10.1016/0014-5793(89)80019-5