Resveratrol as a protective agent of goat sperm submitted to sex sorting in discontinuous Percoll gradient - preliminary results

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Resveratrol as a protective agent of goat sperm submitted to sex sorting in discontinuous Percoll gradient - preliminary results

Background Sexing sperm is an important biotechnical that enables improve animal production, with achievement of maximum animal breeding. However, all sex semen methodologies can promote irreparable damages to sperm cell, which compromise their fertility [1]. Among the main causes of injures to the sperm are the reactive oxygen species, which have their production intensified during semen manip...

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enrichment of undifferentiated type a spermatogonia from goat testis using discontinuous percoll density gradient and differential plating

background: the well documented source for adult multipotent stem cells is spermatogonial stem cells (sscs). they are the foundation of spermatogenesis in the testis throughout adult life by balancing self-renewal and differentiation. the aim of this study was to assess the effect of percoll density gradient and differential plating on enrichment of undifferentiated type a spermatogonia in diss...

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Enrichment of Undifferentiated Type A Spermatogonia from Goat Testis Using Discontinuous Percoll Density Gradient and Differential Plating

BACKGROUND The well documented source for adult multipotent stem cells is Spermatogonial Stem Cells (SSCs). They are the foundation of spermatogenesis in the testis throughout adult life by balancing self-renewal and differentiation. The aim of this study was to assess the effect of percoll density gradient and differential plating on enrichment of undifferentiated type A spermatogonia in disso...

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Discontinuous Percoll gradient preparation for donor insemination: determinants for success.

The use of cryopreserved donor spermatozoa for insemination has become necessary to decrease the risk from sexually transmitted infectious diseases. Lower fecundity rates have been reported with this practice. Efforts have been applied to increase success, including identification of those sperm characteristics which correlate with increased fecundity. Data from in-vitro fertilization have reve...

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Immature germ cell separation using a modified discontinuous Percoll gradient technique in human semen.

The difficulty of identifying immature germ cells in unstained, fresh semen has led most laboratories to use the broad definition 'round cells' to indicate cells other than spermatozoa, thus grouping together both leukocytes and immature germ cells. This is also the case in research andrology, where very little attention has been given to immature germ cells in the semen apart from some rare ex...

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ژورنال

عنوان ژورنال: BMC Proceedings

سال: 2014

ISSN: 1753-6561

DOI: 10.1186/1753-6561-8-s4-p149