Rhamnolipids produced by Pseudomonas : from molecular genetics to the market

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Chemical characterization and physical and biological activities of rhamnolipids produced by Pseudomonas aeruginosa BN10.

Pseudomonas aeruginosa BN10 isolated from hydrocarbon-polluted soil was found to produce rhamnolipids when cultivated on 2% glycerol, glucose, n-hexadecane, and n-alkanes. The rhamnolipids were partially purified on silica gel columns and their chemical structures elucidated by combination of one- and two-dimensional 1H and 13C NMR techniques and ESI-MS analysis. Eight structural rhamnolipid ho...

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Improved Production of Rhamnolipids by a Pseudomonas aeruginosa Mutant

A Pseudomonas aeruginosa mutant derived by random mutagenesis with N-methyl-N¢-nitro-N-nitrosoguanidine, producing high levels of the rhamnolipid biosurfactants was selected on Siegmund-Wagner (SW) plates. The mutant designated P. aeruginosa PTCC1637 produces rhamnolipids at concentrations 10 time more than parent strain. NMR analysis and surface tension measurement showed that the biosurfactan...

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RHAMNOLIPIDS PRODUCED BY PSEUDOMONAS AERUGINOSA GROWN ON ^-PARAFFIN (MIXTURE OF C12, ds AND Cu FRACTIONS)

Two kinds of glycolipids (R-l and R-2) were produced in the culture media by several strains of Pseudomonas aeruginosa when grown on nparaffin (mixture of C12, C13. and Cu fractions). These compounds were isolated through the extraction of the culture broth with ethylacetate and the chromatography on silicic acid column. On the basis of chemical analysis, these lipids were characterized as 2-O-...

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Studies on the Production of Rhamnolipids by Pseudomonas Putida

Rhamnolipid as a potent natural biosurfactant has a wide range of potential applications, including enhanced oil recovery, biodegradation, and bioremediation. Observation of tensio-active indicated that biosurfactants were produced by the newly isolated and promising strain Pseudomonas putida. The biosurfactants were identified as rhamnolipids, the amphiphilic surfaceactive glycolipids usually ...

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Production of rhamnolipids by Pseudomonas chlororaphis, a nonpathogenic bacterium.

Rhamnolipids, naturally occurring biosurfactants constructed of rhamnose sugar molecules and beta-hydroxyalkanoic acids, have a wide range of potential commercial applications. In the course of a survey of 33 different bacterial isolates, we have identified, using a phenotypic assay for rhamnolipid production, a strain of the nonpathogenic bacterial species Pseudomonas chlororaphis that is capa...

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ژورنال

عنوان ژورنال: Microbial Biotechnology

سال: 2020

ISSN: 1751-7915,1751-7915

DOI: 10.1111/1751-7915.13700