Ribosome Display Technology: Applications in Disease Diagnosis and Control
نویسندگان
چکیده
منابع مشابه
Technique review Ribosome display: Cell-free protein display technology
Ribosome display is a cell-free system for the in vitro selection of proteins and peptides from large libraries. It uses the principle of coupling individual nascent proteins (phenotypes) to their corresponding mRNA (genotypes), through the formation of stable protein–ribosome–mRNA (PRM) complexes. This permits the simultaneous isolation of a functional nascent protein, through affinity for a l...
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In this paper, parametric technology display and a mathematical model of technology spillover as a different approach based on the technology changes would be discussed. The recently action that will be accomplished through formation matrices of technological changes, measure of technology spillover and other concepts along with a variety of matrices types. Several examples are given and the re...
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The expression of exogenous peptides on the surface of filamentous bacteriophage was initially described by Smith in 1985. Since his first study, different molecules such as small peptides and antibodies have been displayed on coat proteins of phage, greatly expanding the applications of the technology. The past decade has seen considerable progress in the techniques and applications of phage l...
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Ribosome display is an in vitro evolution technology for proteins. It is based on in vitro translation, but prevents the newly synthesized protein and the mRNA encoding it from leaving the ribosome. It thereby couples phenotype and genotype. Since no cells need to be transformed, very large libraries can be used directly in selections, and the in vitro amplification provides a very convenient i...
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In-vitro display technologies combine two important advantages for identifying and optimizing ligands by evolutionary strategies. First, by obviating the need to transform cells in order to generate and select libraries, they allow a much higher library diversity. Second, by including PCR as an integral step in the procedure, they make PCR-based mutagenesis strategies convenient. The resulting ...
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ژورنال
عنوان ژورنال: Antibodies
سال: 2020
ISSN: 2073-4468
DOI: 10.3390/antib9030028