RISE microscopy: сorrelative Raman-SEM imaging
نویسندگان
چکیده
منابع مشابه
Imaging of endodontic biofilms by combined microscopy (FISH/cLSM - SEM).
Scanning electron microscopy is a useful imaging approach for the visualization of bacterial biofilms in their natural environments including their medical and dental habitats, because it allows for the exploration of large surfaces with excellent resolution of topographic features. Most biofilms in nature, however, are embedded in a thick layer of extracellular matrix that prevents a clear ide...
متن کاملBiological imaging with coherent Raman scattering microscopy: a tutorial.
Coherent Raman scattering (CRS) microscopy is gaining acceptance as a valuable addition to the imaging toolset of biological researchers. Optimal use of this label-free imaging technique benefits from a basic understanding of the physical principles and technical merits of the CRS microscope. This tutorial offers qualitative explanations of the principles behind CRS microscopy and provides info...
متن کاملImaging chemistry inside living cells by stimulated Raman scattering microscopy.
Stimulated Raman scattering (SRS) microscopy is a vibrational imaging platform developed to visualize chemical content of a biological sample based on molecular vibrational fingerprints. With high-speed, high-sensitivity, and three-dimensional sectioning capability, SRS microscopy has been used to study chemical distribution, molecular transport, and metabolic conversion in living cells in a la...
متن کاملVibrational imaging based on stimulated Raman scattering microscopy
A stimulated Raman scattering microscope with near-infrared picosecond laser pulses at high repetition rates (76MHz) and radio-frequency lock-in detection is accomplished. Based on stimulated Raman loss detection, we demonstrate noninvasive point-by-point vibrational mapping of chemical and biological samples with high sensitivity and without the requirement for labeling of the sample with natu...
متن کاملQuantitative chemical imaging with multiplex stimulated Raman scattering microscopy.
Stimulated Raman scattering (SRS) microscopy is a newly developed label-free chemical imaging technique that overcomes the speed limitation of confocal Raman microscopy while avoiding the nonresonant background problem of coherent anti-Stokes Raman scattering (CARS) microscopy. Previous demonstrations have been limited to single Raman band measurements. We present a novel modulation multiplexin...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nanoindustry Russia
سال: 2015
ISSN: 1993-8578
DOI: 10.22184/1993-8578.2015.56.2.50.57