RNA Interference Screen to Identify Pathways That Enhance or Reduce Nonviral Gene Transfer During Lipofection
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چکیده
منابع مشابه
RNA interference screen to identify pathways that enhance or reduce nonviral gene transfer during lipofection.
Some barriers to DNA lipofection are well characterized; however, there is as yet no method of finding unknown pathways that impact the process. A druggable genome small-interfering RNA (siRNA) screen against 5,520 genes was tested for its effect on lipofection of human aortic endothelial cells (HAECs). We found 130 gene targets which, when silenced by pooled siRNAs (three siRNAs per gene), res...
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ژورنال
عنوان ژورنال: Molecular Therapy
سال: 2008
ISSN: 1525-0016
DOI: 10.1038/mt.2008.147