RNA Preparation of Saccharomyces cerevisiae Using the Digestion Method may Give Misleading Results
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RNA Preparation of Saccharomyces cerevisiae Using the Digestion Method may Give Misleading Results
Zymolyase (lyticase) is used for cell wall digestion in yeast experiments and is needed for incubation processes under moderate experimental conditions. This has been thought to cause unfavorable effects, and many researchers are aware that the enzyme method is unsuitable for RNA preparation following several reports of stress responses to the enzyme process. However, RNA preparation with enzym...
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The yeast Saccharomyces cerevisiae has long been an important model organism for biological investigation (1). More recently, high-throughput approaches using deletion libraries and fusion protein libraries have provided powerful techniques with which to study the entire yeast genome and proteome (2–6). However, the methods typically used for preparing high-quality RNA samples from yeast are im...
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ژورنال
عنوان ژورنال: Applied Biochemistry and Biotechnology
سال: 2013
ISSN: 0273-2289,1559-0291
DOI: 10.1007/s12010-012-0051-8