Role of N-terminal extension ofBacillus stearothermophilusRNase H2 and C-terminal extension ofThermotoga maritimaRNase H2
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چکیده
منابع مشابه
Insights into the maturation of hyperthermophilic pyrolysin and the roles of its N-terminal propeptide and long C-terminal extension.
Pyrolysin-like proteases from hyperthermophiles are characterized by large insertions and long C-terminal extensions (CTEs). However, little is known about the roles of these extra structural elements or the maturation of these enzymes. Here, the recombinant proform of Pyrococcus furiosus pyrolysin (Pls) and several N- and C-terminal deletion mutants were successfully expressed in Escherichia c...
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Bacillus amyloliquefaciens a-amylase (1,4-a-D-glucan glucanohydrolase. EC 3.2.1.1), which is commercially supplied as 'Bacillus subtilis a-amylase' does not cross-react immunologically with B. subtilis a-amylase. This enzyme (from B. amyloliquefaciens) was cleaved by treatment with CNBr into seven fragments. Peptide A was selected for sequence determination. It is the longest one, containing 18...
متن کاملMutagenic Analysis of the C-Terminal Extension of Lsm1
The Sm-like proteins (also known as Lsm proteins) are ubiquitous in nature and exist as hexa or heptameric RNA binding complexes. They are characterized by the presence of the Sm-domain. The Lsm1 through Lsm7 proteins are highly conserved in eukaryotes and they form a hetero-octameric complex together with the protein Pat1. The Lsm1-7-Pat1 complex plays a key role in mRNA decapping and 3'-end p...
متن کاملHomology within the N-terminal extension of cysteine proteinases.
It is now well established that the cysteine proteinases of plants (e.g. papain, actinidin, bromelain) and animals (e.g. cathepsins B, H and L) are homologous (see Takio et al., 1983) and almost certainly evolved from a common ancestral protein. For all but one of the cysteine proteinases sequenced to date the N-terminal residues can be exactly aligned with one another at a distance of 25-29 re...
متن کاملMolecular evolution of troponin I and a role of its N-terminal extension in nematode locomotion.
The troponin complex, composed of troponin T (TnT), troponin I (TnI), and troponin C (TnC), is the major calcium-dependent regulator of muscle contraction, which is present widely in both vertebrates and invertebrates. Little is known about evolutionary aspects of troponin in the animal kingdom. Using a combination of data mining and functional analysis of TnI, we report evidence that an N-term...
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ژورنال
عنوان ژورنال: FEBS Journal
سال: 2013
ISSN: 1742-464X
DOI: 10.1111/febs.12479