RP-HPLC stability-indicating method for simultaneous determination of sodium valproate, methylparaben and propylparaben in oral solution

Abstract A new, sensitive, stability-indicating reversed-phase HPLC method was validated and applied for the simultaneous quantitation of sodium valproate two paraben preservatives; methylparaben, propylparaben in liquid dosage form. Stability tests were carried out through exposure analyte's solution to stress conditions. Separation analytes achieved on (waters) C18 Column (150 mm × 3.9 mm, 5 ?m). mixture 0.05 M monobasic potassium phosphate pH 3.5 acetonitrile (50:50; v/v) at 1.5 ml/min flow rate UV detection wavelength 210 nm. The degradation products completely separated. linearity performed range 50–150 % from a target concentration 10 ?g/ml propylparaben, 90 2.88 mg/ml with coefficient correlation (R 2 ) 1.0 valproate. validation results suggested good agreement ICH guidelines. Application proposed analysis forms successfully routine quality control process.