Scorpion: Facilitating High Throughput Electron Microscopy

High-throughput Neurotechnology and Quantum Electron Microscopy

Therapeutic treatment of central nervous system pathologies, such as spinal cord injuries, brain trauma, stroke, and neurodegenerative disorders, will greatly benefit from the discovery of small molecules that enhance neuronal growth after injury. Identification of a diverse repertoire of such molecules and of their cellular targets can also provide important tools for fundamental investigation...

High-throughput Hyperspectral Microscopy

A hyperspectral imager provides a 3-D data cube in which the spatial information (2-D) of the image is complemented by spectral information (1-D) about each spatial location. A static, high-throughput spectrometer design previously developed by our group can be used as the spectral engine in a high-throughput hyperspectral imager that avoids the Fourier undersampling issues present in previous ...

High-throughput nonlinear optical microscopy.

High-resolution microscopy methods based on different nonlinear optical (NLO) contrast mechanisms are finding numerous applications in biology and medicine. While the basic implementations of these microscopy methods are relatively mature, an important direction of continuing technological innovation lies in improving the throughput of these systems. Throughput improvement is expected to be imp...

Semiautomatic, high-throughput, high-resolution protocol for three-dimensional reconstruction of single particles in electron microscopy.

In this chapter we describe the steps needed for reconstructing the three-dimensional structure of a macromolecular complex starting from its projections collected in electron micrographs. The concepts are shown through the use of Xmipp 3.0, a software suite specifically designed for the image processing of biological structures imaged with electron or X-ray microscopy. We illustrate the image ...

Electron Microscopy of Fowl Pox Virus