Screening and Validation of Rice OsAAP6 Interaction Protein

The protein content of rice seeds is an extremely important quality trait, but its genetic basis and molecular regulatory mechanism are still unclear. This study focuses on a positive gene OsAAP6 grain in rice. Proteins that interact with were screened using yeast two hybrid experiments, validated vivo point-to-point experiments bimolecular fluorescence complementarity tests (BiFC). main results as follows: 98 colonies may from cDNA library technology. After sequencing analysis, 40 proteins ultimately obtained. Through comparative three (Pyruvate phosphate dikinase (PPDR), WRKY, GreA) selected these each other. In (BiFC) used to further verify PPDK, GreA can protein, respectively. Therefore, the this will provide clues for revealing by which regulates content.