Screening differentially expressed cDNA clones obtained by differential display using amplified RNA
نویسندگان
چکیده
منابع مشابه
Screening of differentially amplified cDNA products from RNA arbitrarily primed PCR fingerprints using single strand conformation polymorphism (SSCP) gels.
Arbitrarily primed PCR fingerprinting of RNA and differential display resolved on an acrylamide gel has been extensively used to detect differentially expressed RNAs. However, after a differentially amplified product is detected the next steps are labor-intensive: a small portion of the fingerprinting gel that contains the differentially amplified product is cut out, reamplified and the correct...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1997
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/25.4.913