Selected Wheat Seed Defense Proteins Exhibit Competitive Binding to Model Microbial Lipid Interfaces
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چکیده
منابع مشابه
Selected wheat seed defense proteins exhibit competitive binding to model microbial lipid interfaces.
Puroindolines (Pins) and purothionins (Pths) are basic, amphiphilic, cysteine-rich wheat proteins that play a role in plant defense against microbial pathogens. This study examined the co-adsorption and sequential addition of Pins (Pin-a, Pin-b, and a mutant form of Pin-b with Trp-44 to Arg-44 substitution) and β-purothionin (β-Pth) model anionic lipid layers using a combination of surface pres...
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A key molecular event in prion diseases is the conversion of the normal cellular form of the prion protein (PrPC) to an aberrant form known as the scrapie isoform, PrPSc. Under normal physiological conditions PrPC is attached to the outer leaflet of the plasma membrane via a GPI-anchor. It has been proposed that a direct interaction between PrP and lipid membranes could be involved in the conve...
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Published, JBC Papers in Press, October 17, 2005, DOI 10.1074/jbc.R500015200 Thomas Kolter, Florian Winau, Ulrich E. Schaible, Matthias Leippe, and Konrad Sandhoff From the Kekulé-Institut für Organische Chemie und Biochemie, D-53121 Bonn, Germany, the Max-Planck-Institut für Infektionsbiologie, Abteilung Immunologie, D-10117 Berlin, Germany, and the Zoologisches Institut der Universität Kiel, ...
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The time of synthesis, the molecular weight, and the relative glutamine-glutamate and proline to leucine ratios of the endosperm proteins of wheat (Triticum aestivum L. cv. Logan) were determined using a sodium dodecyl sulfate-polyacrylamide gel technique. In general, synthesis of most proteins occurred through much of the maturation of the seed, but past 20 days the rate of synthesis of the hi...
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The competitive adsorption of human serum albumin (HSA), human immuno-gamma-globulin (HIgG) and human fibrinogen (HFb) onto polystyrene (PS) at 20 degrees C and a pH of 7.35 (phosphate-buffered saline) was studied. Protein adsorption was studied using enzyme immunoassay. The results obtained with the immunoassay were compared with those obtained using radiolabelled proteins. Recent studies reve...
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ژورنال
عنوان ژورنال: Journal of Agricultural and Food Chemistry
سال: 2013
ISSN: 0021-8561,1520-5118
DOI: 10.1021/jf401336a