Selenocysteine synthase from Escherichia coli. Analysis of the reaction sequence.
نویسندگان
چکیده
منابع مشابه
Detection of the eaeA Gene in Escherichia coli Isolated from Broiler Chickens by Polymerase Chain Reaction
The aim of this study was to isolate Escherichia coli from chickens and to determine the presence of the eaeA gene, a virulence factor detected in Escherichia coli, in the isolates by polymerase chain reaction (PCR). Different chicken organs (lung, liver and spleen) were inoculated onto blood agar and biochemical tests were performed on the suspicious isolates. Escherichiacoliwas isolated from ...
متن کاملPhylogenetic analysis of Escherichia coli strains isolated from human samples
Escherichia coli (E. coli) is a normal inhabitant of the gastrointestinal tract of vertebrates, including humans. Phylogenetic analysis has shown that E. coli is composed of four main phylogenetic groups (A, B1, B2 and D). Group A and B1 are generally associated with commensals, whereas group B2 is associated with extra-intestinal pathotypes. Most enteropathogenic isolates, however, are assigne...
متن کاملThe nature of the minimal 'selenocysteine insertion sequence' (SECIS) in Escherichia coli.
The UGA codon, usually a stop codon, can also direct the incorporation into a protein of the modified amino acid selenocysteine. This UGA decoding process requires a cis -acting mRNA element called 'selenocysteine insertion sequence' (SECIS) that can form a stem-loop structure. In Escherichia coli the SECIS of the selenoprotein formate dehydrogenase (FdhH) mRNA has been previously described to ...
متن کاملInvestigation of Escherichia coli Selenocysteine Synthase (SelA) Complex Formation Using Cryo-Electron Microscopy (Cryo-EM)
Incorporation of selenocysteine (Sec U) into proteins is directed by a in-frame UGA codon in all domains of life. In Bacteria, Sec biosynthesis and incorporation involves the interaction of Selenocysteine Synthase (SelA), tRNA (SelC or tRNA Sec ), Selenophosphate Synthetase (SPS), a specific elongation factor known as SelB and the specific mRNA structure SElenocysteine Insertion Sequence (SECIS...
متن کاملWobble decoding by the Escherichia coli selenocysteine insertion machinery
Selenoprotein expression in Escherichia coli redefines specific single UGA codons from translational termination to selenocysteine (Sec) insertion. This process requires the presence of a Sec Insertion Sequence (SECIS) in the mRNA, which forms a secondary structure that binds a unique Sec-specific elongation factor that catalyzes Sec insertion at the predefined UGA instead of release factor 2-m...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1991
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)38121-3