Seminal plasma proteins interacting with sperm surface revert capacitation indicators in frozen-thawed ram sperm
نویسندگان
چکیده
منابع مشابه
Equine seminal plasma on preserving the viability of frozen-thawed ram sperm
Re-suspension of frozen-thawed ram sperm with ovine and bovine whole seminal plasma (SP) is beneficial for its post-thawing viability. However, neither the influence of SP incubation duration nor the re-suspension with equine SP has been tested. In the first experiment, frozen-thawed ram sperm were incubated with SP from bovine (BSP), equine (ESP) or ovine (OSP) or without SP (-SP) for short (5...
متن کاملSeminal plasma proteins revert the cold-shock damage on ram sperm membrane.
Ejaculated ram spermatozoa, freed from seminal plasma by a dextran/swim-up procedure and exposed to cold shock, were incubated with ram seminal plasma proteins and analyzed by fluorescence markers and scanning electron microscopy. Seminal plasma proteins bound to the sperm plasma membrane modified the functional characteristics of damaged spermatozoa, reproducing those of live cells. Scanning e...
متن کاملComparison of Different Sperm Selection Techniques in Ram Frozen - Thawed Sperm
Background: The success of fertilization is directly associated with semen quality and the sperm preparation. Considering the common use of cryopreserved spermatozoa, there is a need to develop strategies for sperm preparation in order to achieve a sperm sample of high quality through a rigorous selection of sperm. Thus, sperm cells are being more extensively investigate. This study aimed evalu...
متن کاملProteins implicated in sperm capacitation.
Spermatozoa after being deposited in the female reproductive tract spend a considerable time in this foreign environment prior to fertilization of the oocyte. Chang and Austin independently observed1,2 that this time spent by the spermatozoa in the female tract is not consequential but a necessary event in the life cycle of the male gamete, and Austin2 first called this maturation period of spe...
متن کاملThe effect of seminal plasma on the quality of coated ram frozen-thawed spermatozoa
Two experiments were designed to examine the effects of crude seminal plasma (CSP) exposure ofejaculated and epididymal spermatozoa before freezing. Experiment 1, two consecutive ejaculates werecollected (n=4) within 5 min, the second one was carried out in a tube containing Fiser extender (coatedspermatozoa); by centrifugation, seminal plasma was removed from coated ejaculates. The pellets wer...
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ژورنال
عنوان ژورنال: Animal Reproduction Science
سال: 2016
ISSN: 0378-4320
DOI: 10.1016/j.anireprosci.2016.08.007