Sensitive, Nonradioactive Differential Display Method Using Chemiluminescent Detection
نویسندگان
چکیده
منابع مشابه
Differential Display Method Using Chemi - luminescent Detection
the LCLINK-D assembly. The sluggishness of the VHICH1-LINK-D reaction may be caused by formation of strong secondary structures within the coding region of CHI, which would also account for the relatively weaker amplification of the Fd gene (as compared to the VH gene) as observed previously (9). Although the PCR assembly strategy has been described as somewhat difficult (4), we find it an attr...
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by Northern blot analysis . Figure I shows part of the sequencing gel from the differential display experiments using chemiluminescent detection. Generally, about 100-200 bands were seen from the whole gel for each primer set; this number is about the same as with radioisotope detection (data not shown) ; most of the bands showed identical intensity among the cells examined. The band indicated ...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 1996
ISSN: 0736-6205,1940-9818
DOI: 10.2144/19962003342