Sensitivity of the nested-polymerase chain reaction (PCR) assay for Brugia malayi and significance of ‘free’ DNA in PCR-based assays
نویسندگان
چکیده
منابع مشابه
Polymerase Chain Reaction (PCR)
The polymerase chain reaction (PCR) is a powerful and widely used technique that has greatly advanced our ability to analyze genes. Genomic deoxyribonucleic acid (DNA) present in cells contains many thousands of genes. This makes it difficult to isolate and analyze any individual gene. PCR allows specific DNA sequences, usually corresponding to genes or parts of genes, to be copied from genomic...
متن کاملA rapid and simplified method of DNA extraction for the detection of Brugia malayi infection in mosquitoes by PCR assay.
Currently used protocols for the extraction of filarial parasite DNA from mosquito samples are tedious and involve extensive use of expensive and hazardous chemicals. Therefore, in order to arrive at a simple procedure, four different methods (A, B, C and D) were tried for the extraction of DNA from mosquitoes infected with filarial parasite, Brugia malayi. Method D was found to be as efficient...
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ABSTRACT Recently, PCR is being reported more frequently with satisfactory results on the diagnosis of clinical infections. Widespread availability of PCR promises a sensitive and specific alternative, to traditional methods, but these benefits must be balanced against cost. Protocols using small volumes have described where the reaction taken place in capillary tubes, and small volumes work...
متن کاملDevelopment Application of Polymerase Chain Reaction (PCR)
Polymerase chain reaction (PCR) is an in vitro technique to synthesize large quantities of a given DNA molecule that separates the DNA into two complementary strands, uses a peltier heat pump to quickly heat and cool the DNA and uses the Taq polymerase for the synthesis of DNA. Taq is a bacterium that lives by volcanic sulfer jets at the bottom of the ocean where the temperature is very high. F...
متن کاملComparison of Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) and Agglutination Assays in Diagnosis of Brucellosis in Golestan Province, North of Iran
Introduction: Brucellosis is one of the most common zoonotic infections worldwide. The clinical symptoms of brucellosis are similar to a wide range of diseases; hence, reliable diagnostic and laboratory methods are required to identify the causative agent. Iran is an endemic region of brucellosis, and many patients are misdiagnosed due to the nature of the infection. In this study, we aimed to ...
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ژورنال
عنوان ژورنال: International Journal for Parasitology
سال: 2000
ISSN: 0020-7519
DOI: 10.1016/s0020-7519(00)00104-1