Separation of ribosomal subunits on agarose gels
نویسندگان
چکیده
منابع مشابه
Apolipoprotein E genotyping on agarose gels.
References 1. Young DS, Bermes EW Jr. Specimen collection and processing-sources of biological variation. In: Burtis CA, Ashwood ER, eds. Tietz textbook of clinical chemistry, 2nd ed. Philadelphia: WB Saunders, 1994:58-101. 2. Lahiri DK, Schnabeh B. DNA isolation by a rapid method from human blood samples: effects of MgC12, EDTA, storage time, and temperature on DNA yield and quality. Biochem G...
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cloning could be achieved by using this quick, alternative protocol. Not only does this Na3VO4-inhibition procedure save time, but also note that in this protocol the adjustment of vector-to-insert ratio in the ligation reaction is not necessary. To ligate with the CIAP/Na3VO4-treated vector (ca. 50 ng), we recommend using as much excess insert as is practical. Eliminating the quantitation step...
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A new agarose-based protein electrophoresis gel system is described. The system consists of a highly resolving agarose, MetaPhor XR (FMC BioProducts, Rockland, ME, USA) dissolved in urea and TBE buffer and a stacking gel composed of a high gel-strength agarose, SeaKem Gold (FMC BioProducts). TBE containing sodium dodecyl sulfate (SDS) is used as electrophoresis buffer. The disadvantages of trad...
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Nucleic acids that have been subjected to electrophoresis through agarose gels may be detected by staining and visualized by illumination with 300-nm UV light. Methods for staining and visualization of DNA using either ethidium bromide or SYBR Gold are described here. The most convenient and commonly used method to visualize DNA in agarose gels is staining with the fluorescent dye ethidium brom...
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A method that provides an easy, rapid, and reproducible way for separating closed circular DNA species from linear DNA and nicked circles is described. The method is based on the difference in mobility of form I (supercoiled) DNA and form II (nicked circles), and the differential mobility of relaxed circular DNA in the presence and absence of ethidium bromide (EtdBr). It can be used for detecti...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1980
ISSN: 0014-5793
DOI: 10.1016/0014-5793(80)80066-4