Sequence analysis of cereal sucrose synthase genes and isolation of sorghum sucrose synthase gene fragment
نویسندگان
چکیده
منابع مشابه
Isolation and sequencing of tomato fruit sucrose synthase cDNA.
Suc synthase (EC 2. 4. 1. 13) catalyzes the reversible reaction: Suc + UDP c-, UDP-Glc + Fru. In plants, its main physiological function is to cleave Suc and generate UDPGlc, which is used in such diverse pathways as starch synthesis, cell wall component synthesis, and energy generation (Huber and Akazawa, 1986; Sung et al., 1988). It has been demonstrated that Suc synthase is a key enzyme duri...
متن کاملSucrose synthase of soybean nodules.
SUCROSE SYNTHASE (UDPGLUCOSE: d-fructose 2-alpha-d-glucosyl transferase, EC 2.4.1.13) has been purified from the plant cytosolic fraction of soybean (Glycine max L. Merr cv Williams) nodules. The native enzyme had a molecular weight of 400,000. The subunit molecular weight was 90,000 and a tetrameric structure is proposed for soybean nodule sucrose synthase. Optimum activity in the sucrose clea...
متن کاملSucrose phosphate synthase and sucrose accumulation at low temperature.
The influence of growth temperature on the free sugar and sucrose phosphate synthase content and activity of spinach (Spinacia oleracea) leaf tissue was studied. When plants were grown at 25 degrees C for 3 weeks and then transferred to a constant 5 degrees C, sucrose, glucose, and fructose accumulated to high levels during a 14-d period. Predawn sugar levels increased from 14- to 20-fold over ...
متن کاملCircadian Regulation of Sucrose Phosphate Synthase Activity
Sucrose phosphate synthase (SPS), a key enzyme in sucrose biosynthesis, is regulated by protein phosphorylation and shows a circadian pattern of activity in tomato. SPS is most active in its dephosphorylated state, which normally coincides with daytime. Applying okadaic acid, a potent protein phosphatase inhibitor, prevents SPS activation. More interesting is that a brief treatment with cyclohe...
متن کاملPurification of Endosperm Sucrose Synthase From Guichao
Sucrose synthase activity in rice is potentially yield limiting. Earlier research indicated that endosperm sucrose synthase activity during grain filling was higher in ‘Guichao’ rice than in U.S. rice cultivars. We set about to purify sucrose synthase from Guichao endosperm as part of an overall research effort to understand the physiological reasons for the high yields of Guichao compared to U...
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ژورنال
عنوان ژورنال: African Journal of Biotechnology
سال: 2007
ISSN: 1684-5315
DOI: 10.5897/ajb2007.000-2374